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Syngas Biofuels Energy Inc electrotransformation generator
Electrotransformation Generator, supplied by Syngas Biofuels Energy Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/electrotransformation+generator/electrotransformation+generator/pm23775000-283-21-10
Average 90 stars, based on 1 article reviews
electrotransformation generator - by Bioz Stars, 2026-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: Expression of amplified synthetic ethanol pathway integrated using Tn7-tool and powered at the expense of eliminated pta, ack, spo0A and spo0J during continuous syngas or CO2 /H2 blend fermentation.
Article Snippet: Aims: To engineer acetogen biocatalyst selectively overproducing ethanol from synthesis gas or CO2/H2 as the only liquid carbonaceous product.. Methods and Results: Ethanol-resistant mutant originally capable of producing only acetate from CO2/CO was engineered to eliminate acetate production and spore formation using our proprietary Cre-lox66/lox71-system.. Bifunctional aldehyde/alcohol dehydrogenase was inserted into the chromosome of the engineered mutant using Tn7based approach.

Article Title: Synthetic 2,3-butanediol pathway integrated using Tn7-tool and powered via elimination of sporulation and acetate production in acetogen biocatalyst.
Article Snippet: Acetogen Clostridium sp. MT1802 originally producing 336-mM acetate from inorganic carbon of CO2/CO was engineered to eliminate acetate production and sporulation using Cre-lox66/lox71-approach.. The recombinant started producing 105-mM formate expressing synthetic formate dehydrogenase integrated in two copies.. Formate-producing recombinant was further engineered to express synthetic formate acetyltransferase, acetolactate synthase, acetolactate decarboxylase, and alcohol dehydrogenase integrated in two copies each using Tn7 tool.

Article Title: Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.
Article Snippet: Methanol-resistant mutant acetogen Clostridium sp. MT1424 originally producing only 365 mM acetate from CO2/CO was engineered to eliminate acetate production and spore formation using Cre-lox66/lox71-system to power subsequent methanol production via expressing synthetic methanol dehydrogenase, formaldehyde dehydrogenase and formate dehydrogenase, three copies of each, assembled in cluster and integrated to chromosome using Tn7-based approach.. Production of 2.2 M methanol was steady (p\ 0.005) in single step fermentations of 20 % CO2 ?. 80 % H2 blend (v/v) 25 day runs each in five independent repeats.

Article Title: Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend.
Article Snippet: Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation.. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO2/H2 blend.. Acetate production and spore formation were eliminated from Clostridium sp. MT1243 using Cre-lox66/lox71-system.

Blocking Assay:

Article Title: Expression of amplified synthetic ethanol pathway integrated using Tn7-tool and powered at the expense of eliminated pta, ack, spo0A and spo0J during continuous syngas or CO2 /H2 blend fermentation.
Article Snippet: Aims: To engineer acetogen biocatalyst selectively overproducing ethanol from synthesis gas or CO2/H2 as the only liquid carbonaceous product.. Methods and Results: Ethanol-resistant mutant originally capable of producing only acetate from CO2/CO was engineered to eliminate acetate production and spore formation using our proprietary Cre-lox66/lox71-system.. Bifunctional aldehyde/alcohol dehydrogenase was inserted into the chromosome of the engineered mutant using Tn7based approach.

Article Title: Synthetic 2,3-butanediol pathway integrated using Tn7-tool and powered via elimination of sporulation and acetate production in acetogen biocatalyst.
Article Snippet: Acetogen Clostridium sp. MT1802 originally producing 336-mM acetate from inorganic carbon of CO2/CO was engineered to eliminate acetate production and sporulation using Cre-lox66/lox71-approach.. The recombinant started producing 105-mM formate expressing synthetic formate dehydrogenase integrated in two copies.. Formate-producing recombinant was further engineered to express synthetic formate acetyltransferase, acetolactate synthase, acetolactate decarboxylase, and alcohol dehydrogenase integrated in two copies each using Tn7 tool.

Article Title: Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.
Article Snippet: Methanol-resistant mutant acetogen Clostridium sp. MT1424 originally producing only 365 mM acetate from CO2/CO was engineered to eliminate acetate production and spore formation using Cre-lox66/lox71-system to power subsequent methanol production via expressing synthetic methanol dehydrogenase, formaldehyde dehydrogenase and formate dehydrogenase, three copies of each, assembled in cluster and integrated to chromosome using Tn7-based approach.. Production of 2.2 M methanol was steady (p\ 0.005) in single step fermentations of 20 % CO2 ?. 80 % H2 blend (v/v) 25 day runs each in five independent repeats.

Article Title: Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend.
Article Snippet: Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation.. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO2/H2 blend.. Acetate production and spore formation were eliminated from Clostridium sp. MT1243 using Cre-lox66/lox71-system.

Sterility:

Article Title: Expression of amplified synthetic ethanol pathway integrated using Tn7-tool and powered at the expense of eliminated pta, ack, spo0A and spo0J during continuous syngas or CO2 /H2 blend fermentation.
Article Snippet: Aims: To engineer acetogen biocatalyst selectively overproducing ethanol from synthesis gas or CO2/H2 as the only liquid carbonaceous product.. Methods and Results: Ethanol-resistant mutant originally capable of producing only acetate from CO2/CO was engineered to eliminate acetate production and spore formation using our proprietary Cre-lox66/lox71-system.. Bifunctional aldehyde/alcohol dehydrogenase was inserted into the chromosome of the engineered mutant using Tn7based approach.

Article Title: Synthetic 2,3-butanediol pathway integrated using Tn7-tool and powered via elimination of sporulation and acetate production in acetogen biocatalyst.
Article Snippet: Acetogen Clostridium sp. MT1802 originally producing 336-mM acetate from inorganic carbon of CO2/CO was engineered to eliminate acetate production and sporulation using Cre-lox66/lox71-approach.. The recombinant started producing 105-mM formate expressing synthetic formate dehydrogenase integrated in two copies.. Formate-producing recombinant was further engineered to express synthetic formate acetyltransferase, acetolactate synthase, acetolactate decarboxylase, and alcohol dehydrogenase integrated in two copies each using Tn7 tool.

Article Title: Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.
Article Snippet: Methanol-resistant mutant acetogen Clostridium sp. MT1424 originally producing only 365 mM acetate from CO2/CO was engineered to eliminate acetate production and spore formation using Cre-lox66/lox71-system to power subsequent methanol production via expressing synthetic methanol dehydrogenase, formaldehyde dehydrogenase and formate dehydrogenase, three copies of each, assembled in cluster and integrated to chromosome using Tn7-based approach.. Production of 2.2 M methanol was steady (p\ 0.005) in single step fermentations of 20 % CO2 ?. 80 % H2 blend (v/v) 25 day runs each in five independent repeats.

Article Title: Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend.
Article Snippet: Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation.. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO2/H2 blend.. Acetate production and spore formation were eliminated from Clostridium sp. MT1243 using Cre-lox66/lox71-system.

Electroporation:

Article Title: Expression of amplified synthetic ethanol pathway integrated using Tn7-tool and powered at the expense of eliminated pta, ack, spo0A and spo0J during continuous syngas or CO2 /H2 blend fermentation.
Article Snippet: Aims: To engineer acetogen biocatalyst selectively overproducing ethanol from synthesis gas or CO2/H2 as the only liquid carbonaceous product.. Methods and Results: Ethanol-resistant mutant originally capable of producing only acetate from CO2/CO was engineered to eliminate acetate production and spore formation using our proprietary Cre-lox66/lox71-system.. Bifunctional aldehyde/alcohol dehydrogenase was inserted into the chromosome of the engineered mutant using Tn7based approach.

Article Title: Synthetic 2,3-butanediol pathway integrated using Tn7-tool and powered via elimination of sporulation and acetate production in acetogen biocatalyst.
Article Snippet: Acetogen Clostridium sp. MT1802 originally producing 336-mM acetate from inorganic carbon of CO2/CO was engineered to eliminate acetate production and sporulation using Cre-lox66/lox71-approach.. The recombinant started producing 105-mM formate expressing synthetic formate dehydrogenase integrated in two copies.. Formate-producing recombinant was further engineered to express synthetic formate acetyltransferase, acetolactate synthase, acetolactate decarboxylase, and alcohol dehydrogenase integrated in two copies each using Tn7 tool.

Article Title: Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.
Article Snippet: Methanol-resistant mutant acetogen Clostridium sp. MT1424 originally producing only 365 mM acetate from CO2/CO was engineered to eliminate acetate production and spore formation using Cre-lox66/lox71-system to power subsequent methanol production via expressing synthetic methanol dehydrogenase, formaldehyde dehydrogenase and formate dehydrogenase, three copies of each, assembled in cluster and integrated to chromosome using Tn7-based approach.. Production of 2.2 M methanol was steady (p\ 0.005) in single step fermentations of 20 % CO2 ?. 80 % H2 blend (v/v) 25 day runs each in five independent repeats.

Article Title: Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend.
Article Snippet: Naturally mevalonate-resistant acetogen Clostridium sp. MT1243 produced only 425 mM acetate during syngas fermentation.. Using Clostridium sp. MT1243 we engineered biocatalyst selectively producing mevalonate from synthesis gas or CO2/H2 blend.. Acetate production and spore formation were eliminated from Clostridium sp. MT1243 using Cre-lox66/lox71-system.



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